We narrowed to 582 results for: BASE

...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

...reporters have limited use in vivo because they are based on light which is very easily scattered by tissue...

... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...

...could reveal something fascinating. Behavior is based primarily on careful observation of what an animal...

...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,...

...WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations...

...Think a university system who develops evidence-based university policy to combat sexual harassment in...

...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...

...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...

...interest.  Determine injection titers and volumes based on the serotype Since each serotype also exhibits...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...

...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...

.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...

...PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins...

...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...

...using the virus. You may need to adjust coordinates based on your own method of identifying bregma but it ...