...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...

...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...

.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...

...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...

...PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins...

...using the virus. You may need to adjust coordinates based on your own method of identifying bregma but it ...

... to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

... CasPEDIA is meant to be a constantly updating database which serves as a resource for the scientific ...properties (Makarova et al). CasPEDIA is an evolving database, and all researchers are invited to contribute..., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional Classification System for Class ...

...exhaustive, so you may want to consult the REBASE database for more detailed information. Enzyme Dam...regulating genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis...

..., it should have a corresponding entry in your database. Enjoy your happy lab staff. Barcoded materials...even be worth it to have extra columns in your database to the effect of `dev_name` and `science_name`... database...

...cell lines on public databases. Image attributions (from top to bottom) Database Center for Life Science... Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...

... through comparisons with a reference sequence database. Through a series of simulations and machine learning...identify synthetic and natural sequences in Addgene’s database. They showed a growing trend of sourcing genes...

... the locations where the enzymes will cut (in basepairs), and the total number of times they cut. Use ...nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence...

... mentoring at Mentornet.net. This is an online database of mentors you can tap for email discussions. ...who enter their information in these searchable databases are usually offering their contact information...

...New York University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University...different places have very different cultures. In Basel, I felt that the time flows much slower, like the...

...still growing our "Articles Citing This Plasmid" database - and we always appreciate any help we can get...

...could exist in nature. To do this, they mined a database of microbial genome and metagenome from the Department...

...companies need to pay to obtain them. There’s a database of labels on the My Green Lab website, but if ...