We narrowed to 534 results for: CAR

1. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...

2. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ... the western blot. When planning a western, take care to include the proper positive and negative controls...

3. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...restriction digest and ligation process, you should carefully choose your backbone and insert - these both must...

4. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ...protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and limitations...

5. Antibodies 101: Validation

   Type

   Blog Post

   Published

   March 24, 2022, 1:15 p.m.

   ...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

6. Antibodies 101: Antibody Engineering and Directed Evolution

   Type

   Blog Post

   Published

   Aug. 5, 2025, 1:15 p.m.

   ...References and Resources References Chan, A. C., & Carter, P. J. (2010). Therapeutic antibodies for autoimmunity...

7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...system is to use a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda...

8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ... PMCID: PMC5312033. 2. Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A,...

9. Antibodies 101: Introduction to Antibodies

   Type

   Blog Post

   Published

   Jan. 19, 2021, 2:15 p.m.

   ...: Immunofluorescence staining for CD31 (green), CARD14 (red), and pNFkB (blue) in lesional skin from a...

10. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...fluorophores and their emission spectra. Some fluorophores carry their emission peaks within their names, for example...

11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...strategies are presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents...

12. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

13. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...editing is precise and versatile, but it requires careful optimization to achieve the best efficiency. The...

14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded...