We narrowed to 568 results for: CHI

1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...

2. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...

3. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

4. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

6. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   ... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...

7. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

8. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...

9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

10. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It was also ...

11. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...studying epigenetics routinely use the chromatin IP (ChIP) technique to identify DNA binding proteins involved...

12. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...benefit when recruiting patients, specifically children and patients with skin disorders for research ...

13. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...rewarding parts of the job. Solutions may also be achieved by simply replacing the product in question. This...

14. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ... cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages...

15. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims should have short titles,...

16. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...

17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ...strain distribution centers in the US, Europe, and China. In addition, the Zebrafish Model Organism Database...

18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...vectors, such as adeno-associated virus (AAV), from reaching their targets. The AAV9 serotype can breach the...

19. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out two Bdnf...

20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica...