We narrowed to 553 results for: ESC
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TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...
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New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts Resources... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m.... on a new feature. We start with a story that describes the feature and what the criteria is for accepting... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m...., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m.... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....free of contamination and match their original description. The oldest, largest, and most diverse of these... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....don't see protein overexpression? The strains described above should generate sufficient expression levels... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m.... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m.... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model.... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...