We narrowed to 782 results for: INA
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  TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....beamsplitter, and a 495/30 nm emission filter. This combination results in a 2.6-fold increase in signal relative...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging...
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  Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction toolsTypeBlog PostPublishedJune 27, 2018, 1:16 p.m....specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase...Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from...
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  CRISPR Challenges: Standardization and Homology Directed RepairTypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....notes, since the conference, I think I may have finally grasped just how complicated DNA repair pathways...genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson...
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  Antibodies 101: Avoiding the Mouse on Mouse Mess in IHCTypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....will help get your experiments back on track. One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene ...
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  Multifunctional Neural Fibers: Modulation from Gut to BrainTypeBlog PostPublishedAug. 31, 2023, 1 p.m....– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...
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  Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?TypeBlog PostPublishedJune 9, 2016, 2:30 p.m.... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...
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  Plasmids 101: A Brief History of Plasmids and an Improved eBook!TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored... in areas such as bacterial conjugation and recombination, replication and topology, and cloning and gene...
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  Don’t Wait To Launch Your Health Science StartupTypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....critical three letters after your name (P-H-D). Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we...
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  Even more elegant: Single injection CRISPR/Cas9 in C. elegansTypeBlog PostPublishedJuly 7, 2015, 3:36 p.m....Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now...elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein...
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  Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...
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  Extracurricular Activities for a Strong Science Career PathTypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend...
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  Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica SystemTypeBlog PostPublishedJune 4, 2019, 1:02 p.m....so that it would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation... newly developed green serotonin sensor). In preliminary studies with the lab’s collaborators, they were...
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  Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmidsTypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....another company and catalog number changes) the original identifier loses its meaning, making it difficult...click on the link it will take you directly to the original article online. It is also designed to be persistent...
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  Virtual Science Conference Coming Up? Three Addgenies Share Their ExperienceTypeBlog PostPublishedJune 30, 2020, 1:15 p.m....presenter had four “slides” to use for their poster, eliminating the need to zoom and scroll around the poster...4MysvpzwkP — Samuel Guay (@SamGuay_) June 18, 2020 Final thoughts on virtual conferences Virtual conferences...
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  "Build Your Plasmid": The Game - Play to TeachTypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly...
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  The Importance of High Titer for AAV TransductionsTypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...
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  Multiplexed Overexpression of Regulatory Factors (MORF) collectionTypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....clusters of individual cells along with color coded nominated reference cell types. (Adapted from Joung et ...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...
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  Pushing the Limits of DNA AssemblyTypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded...
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  Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein BiosensorsTypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin...can then be used to determine the amount of HMO originally in the sample. The second sensor is a fusion ...
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  How Dry Ice Affects Viral Vector Sample pH & How to Avoid ItTypeBlog PostPublishedJune 20, 2017, 2:30 p.m....be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...