We narrowed to 771 results for: INA
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TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...
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Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....clusters of individual cells along with color coded nominated reference cell types. (Adapted from Joung et ...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....presenter had four “slides” to use for their poster, eliminating the need to zoom and scroll around the poster...4MysvpzwkP — Samuel Guay (@SamGuay_) June 18, 2020 Final thoughts on virtual conferences Virtual conferences... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....another company and catalog number changes) the original identifier loses its meaning, making it difficult...click on the link it will take you directly to the original article online. It is also designed to be persistent... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....so that it would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation... newly developed green serotonin sensor). In preliminary studies with the lab’s collaborators, they were... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin...can then be used to determine the amount of HMO originally in the sample. The second sensor is a fusion ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....supportive environment to build my management skills. Finally, developing emotional intelligence is vital. Learning...collaboration. The good news: It’s a journey, not a final choice Management is not a one-way path. As careers... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....website, as an instructive step-by-step guide. Finally, when writing your alt text, use all of this information... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy. Type of Virus Pros Cons ... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....dCas9 fused to the Activation Induced Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...norovirus RNA), the ribosome binding site is inaccessible. When the target RNA is present, however, it... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....writing grant proposals to be reviewed by interdisciplinary panels. And highly successful scientists ... graduate students in different regional and disciplinary communities gather at ComSciCon's local and ...