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...other important binding events take place during the course of infection. These binding events can be impaired...infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult...possible. One problem that often plagues labs and hinders experiments is mycoplasma contamination. Mycoplasma...Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All ...

...nose-poke at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, .... If activation of the circuit is sufficient to induce reinforcement (instills a sense of “reward”), then...software such as EthoVision. Consult the literature to find all the applications of optogenetics relevant to...region may affect the animal; stimulation could induce seizures or some other unwanted phenomenon or it...learn optogenetics is straight through the source - find a lab within your own neuroscience sub-discipline...

...hero (that I’m not), FRET loves playing “live.” Indeed, FRET was one of the first techniques which enabled...Historically, molecular interactions were detected by indirect means often using probes with the potential to...may have to design several different constructs to find one that could be used for the purpose of your studies.... But it is a parameter that you have to keep in mind when analysing FRET results. The cross-talk between...also don’t have any resonance transfer. You have to find a balance between FRET efficiency and cross-talk...

...University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting...efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are... et al 2004, Kim et al 2008). While the biology behind this AAV effect remains unclear, it is likely not...infect recipient cells. After G418 selection, individual gene edited clones are identified by PCR. Then...Resources on Addgene.org Check out our Viral Service Find AAV Vectors Browse all Viral Vectors ...

... Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview...before tailoring each presentation appropriately.  Find out who will be listening.  Are they early, mid,...explanation. The people that need the information will find it. Experienced speakers present useful protocols...

... of E. coli used today are descended from two individual isolates, the K-12 strain and the B strain. K...used in the lab Most of the commercial strains you find today are marketed for a specific purpose: fast ...conjugation. Genes listed on F´ are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...

...’t quite seem to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody...very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful. If...place to start looking for answers.    We hope you find the latest addition to our eBook collection useful...

...Addgene.org Check out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian ...including one loxP site insertion and one NHEJ-repaired indel, often occur. Figure 1: In conventional gene...recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in...al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with most projects...

...Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular...Chapter 8 - The importance of communicating research findings through social media and other nontraditional ...

...Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create...listen to the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History ...story telling following the unfortunate story of Rosalind Franklin and her incredibly important role in ... Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids 101 Series...

...red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you...nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab...ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny...

...Researchers in developing countries often find themselves on the front lines of emerging global challenges...and Africa, but we need to expand our efforts to find new and creative ways to reach developing countries..., as well as shine a light on some of the great minds working in developing countries. As the world continues...and organizations like it. Additional Resources Find out how you can get involved with Seeding Labs Follow...

...widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists.... The ends of the branches contain the antigen binding sites and are highly variable, allowing exquisite...The immortalized cells, called hybridomas, are individually isolated and expanded. The clonal cells are ...

...in a variety of cell types, but you should try to find the best AAV for your particular experiment! Traveling...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) ...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001):...Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use AAV...

...calcium binding protein) fused to two fluorescent proteins. When the responsive protein binds to calcium...Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux VNT responds...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...

...expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous...direct modification of DNA within E. coli and is independent of restriction sites. The lambda red system is...with a higher efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA...Resources on Addgene.org Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other ...

... used during the purification procedure. Good indicators of DNA purity are the absorbance measurements...nm should fall ~1.8 - 2 and give scientists an indication of purity versus protein contaminants. The 260...nm ratios should fall ~2 - 2.2, and provide an indication of purity versus chaotropic agents as guanidine...Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

... down What happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our...our three most recent blog posts. Finally, you’ll find everyone’s favorite section: Tools of the Day. Our... obvious ones to discover as you browse. You may find one of your most frequently used buttons stands ...faster to skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to ...

...Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...

...) suggestions for defining the problem and for finding solutions. Even better than just reading this article...Berkeley and Harvard. Students also partner with independent career organizations to get the grants and programs...There are many academic administrators who are finding ways to provide new types of career preparedness...years. However, some organizations are woefully behind and some just don't have the resources to do enough...