We narrowed to 784 results for: IRE;

...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

...and control elements. Development of this tool required creating a database of common features, and devising...Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity when detecting a...

...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...

...down viral genomes containing only the sequences required for viral replication and transcription as well...binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions to your DNA...

...yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

... public and retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures...

...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

...turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about our paper...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

...antibody tool and therefore will grow to serve the entire scientific community.     Why recombinant antibodies.... Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the creation ...

...tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter sounds...34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression systems...

...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...such as transcription factors tethered to dCas9, require long time scales with constitutively expressed ...

...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

...serotonin. Using a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput...anxiety, but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger...

... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

... research projects to a halt. None of us works directly with viruses or transmissible diseases, so we ...and availability; 3) Make the contact to start a direct collaboration between researcher and expert; 4)...

...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research...Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection ...

...ml This method is quick and simple and doesn’t require any special reagents. However, there’s limited ...absorbance-based methods, fluorescence-based methods require a standard curve, a set of samples with a known...

...audience engaged. They first sent a pre-workshop questionnaire to the participants to learn about what they...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...