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We narrowed to 811 results for: LARS

Showing: 521 - 540 of 811 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...cell type? You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  2. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...genes, tags, truncations, and codon optimizations similar to those found in the bacterial expression plasmids...Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated...SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 https://doi....

  3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...and not just the subset of those that return a particular result. Anything else that occurs during the ...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...Promotion Guidelines; preregistration to increase clarity in study design and analysis; removing publication...

  4. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... almost all of the attendees were going to be molecular biologists. This was not at all the case. The ...neurobiology (and really in all of biology) require large data sets. Whether these data sets include videos... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...unsure what your population will look like for a particular marker, you can use the reference plots provided...isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined...and then add in your isotype control for that particular marker. This enables you to identify any non-...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...offset due to the nanobody-GFP interaction). The modularity of the system provides researchers better flexibility...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...

  7. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...for either tissues or cells. The process is very similar for both, though tissue samples do require an extra...thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s all the fuss...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  8. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...used to investigate the effects of activating particular signaling pathways in specific cell types (typically...typically neurons). While all of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double...animals, where cre expression is restricted to a particular cell type. The human synapsin promoter further...

  9. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...Fig. 1B). Tracers are typically delivered to a particular location and they either trace downstream from..., you can map what regions are signaling to a particular site while also activating or inhibiting gene...these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The...

  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...could code the best operations software possible. Similarly, when the developers needed to know information...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...Technologies for the North America region. She is particularly interested in DNA sequencing tech, and the intersection...

  11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to... (HypaCas9). This nuclease showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives...

  12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...

  14. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...worry about getting support from depositors. We regularly hear how much they see the value in depositing...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing... I checked we were ranked as the 21,806th most popular website in the world. I know, right? Upgrades to...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...Check out our molecular biology protocols Find protocol videos on our website Read our Molecular Biology Guide...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

  16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...packaging capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists...

  17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...or other synthetic biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

  18. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise it would be ...achieve!) Each field has its own framework: a particular way of organizing and connecting information ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...Support Team here at Addgene, so troubleshooting molecular biology experiments was in the job description...fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving...result of resolving the issue. Depending on the particular problem, you might even be expected to write ...

  20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...
Showing: 521 - 540 of 811 results