We narrowed to 794 results for: Mpl

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two sites within the viral genome...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...

... the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was able to...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...

...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest starts...

...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual...volunteered for various tasks as things came up. For example, I updated some sections of the NPA’s website. ...

... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...

...specific type of virus you are trying to make. For example, bacteriophages will need to be produced in bacteria... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Processing of your baculovirus is straightforward — simply spin down the supernatant in a centrifuge to remove...

... in proximity by the looping of chromatin, the complex of DNA and proteins that forms chromosomes. These... of the CAPTURE method. After protein and DNA complexes are cross-linked, DNA is digested into smaller...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ...

... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...which multiplies every 16 hours). So if you, for example, split cells on a Monday diluting them 1:10, you...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...

...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...alternative PAM sequences are longer and often more complex than NGG, resulting in lower overall genome frequency... be less predictable than SpCas9 off-targets. Employing xCas9 for transcriptional regulation and base ...

...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown... of different pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to...

...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...for the foreseeable future. These newer findings simply add to our understanding of how this antibody works...

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

...happy to upload your newest resources. Here is an example of a plasmid with a downloadable protocol listed...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...

...allow people to, for example, knock out a gene with simultaneous complementation, with a knockout and ...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs, databases...consistently run our blastx searches with the “Low complexity regions” filter unchecked so that these regions...benefit is that blastp searches do not filter low complexity regions by default, meaning that you do not have...

...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...