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We narrowed to 729 results for: NSI;

Showing: 521 - 540 of 729 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...technical roles. Therefore, institutions are responsible for ensuring that either all affected scientists...and Brandeis universities have stated that they consider postdocs paid on stipends to be FLSA exempt, and...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...gain insight into protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...d like to manipulate in mind. Now it’s time to consider where you would like to attack this gene. Should...sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4; brighter than...

  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...great model organism because they are easy and inexpensive to grow and maintain in the lab, have a short... worm, Caenorhabditis elegans, has been used extensively as a model organism since the 1970s when Sydney...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...this interaction will give you an idea of how responsive and supportive the ELN team is. Once you’ve made...information. If an employee leaves the institution, the transition is smooth as all data is organized within an ...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid dimer....

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...into the mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target ... Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic...

  12. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... that have negative impacts on fitness. One consideration when making a transgenic cell line with the ...the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system can...

  13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in...from a single injected animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...training. Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These... having sufficient data to write a manuscript. Consider questions like: What figures are missing? For ...

  17. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...contacting [email protected]. These deposits are considered large deposits and are exceptions to our standard...and Christina Mork in February 2024.  Additional Inside Addgene Content on the Blog Quickest Way to Deposit...

  18. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers most things CRISPR - from...
Showing: 521 - 540 of 729 results