We narrowed to 629 results for: Ncl

1. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...NHEJ has a vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...Further Reading: For more information about SAM, including a tool for getting optimized guide sequences for...

3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ...Mike recommends that the team should initially include a visionary, a science-minded individual, and a...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...for one or both of these criteria. If you can, include people from different labs, departments or organizations...

5. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ... 4) Modifications made to antibodies, such as inclusion of non-canonical amino acids or peptide tags. ...

6. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...once the ligand is removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible...

7. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the...

8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered "natural tumor...

9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the final read out of this ...

10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...cancelling plans are going ahead with raises anyway, including the entire faculty of the biochemistry department...

11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ... currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated...

12. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...to nine proteins in ten ddifferent cell types, including difficult-to-manipulate primary cells, achieving...

13. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...skills that Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking...

14. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ... for RaPID experiments RaPID negative controls include both scrambled RNA motifs and cells transfected...

15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids, and a constrained...

16. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...Annotate: When creating important documentation, including SOPs or project-related documents, we define new...

17. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ... was acknowledged in hundreds of publications including Cell, Nature, and other journals.   Paolo Colombi...

18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...decrease these so-called “off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ... Someone could take on an engineering project including hundreds of genes and regulation and resource ...

20. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...This has been used for a wide range of purposes, including activating an oncogene only in a particular organ...