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We narrowed to 790 results for: Por

Showing: 521 - 540 of 790 results

  1. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  2. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

  9. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  11. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  13. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  15. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...reader would glean from the image itself.  As we incorporated alt text into our work, we began to realize ..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...the target cell or transiently expressed. It’s important to note that for all the applications we discuss... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...

  17. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... provides a useful yet low-pressure teaching opportunity for lab members or students new to protein expression...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...
Showing: 521 - 540 of 790 results