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We narrowed to 809 results for: Ran;

Showing: 521 - 540 of 809 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... avidin, proximity labelling can capture weak/transient protein interactions as well as be used for both...proximity labeling by promiscuous biotin ligases (Branon et al., 2018). BioID and BioID2 can take hours ...2019). This technique relies on an S. auerus transpeptidase sortase A variant (mgSrtA), which is capable...motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity labeling ...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...dDetermined as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  3. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...looking, I found an inexpensive HDMI transmitter that could transmit a signal between a wall. There was ... things first was getting that wireless HDMI transmitter/receiver, to beam the camera’s image to my remote...then set up a monitor connected to the wireless transmitter and an iPad in the office next door (connected...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...While this may effectively serve as a transfection or transduction control, a different gRNA would have..., you’ll want to select a Cas enzyme with low tolerance for mismatches within the gRNA sequence. ...near each other, this will result in a DNA double strand break (DSB), the event required for frameshift ...

  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...don’t tell us everything there is to know is a strange and exciting revelation. Science in any field can...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens...1-2 years) Often a gap-year position taken to transition to the next career stage (ex: grad school, med...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery, chromosomal...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...

  7. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Although white light is entering your hand, only orange/red light can be seen exiting through the tissue...detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment,...Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT/STED) also excel on these types of samples...when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation and...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... modified cells are transferred into an animal and the behavior of the transferred cells is followed. ...generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target...pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter then showed...

  9. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University of Agriculture...-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects... transgenic crops...

  10. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...choices), we'll be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced you...out to you and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week...their new friend…  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed ...

  12. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...characterized by single, linear, negative-sense, single-stranded RNA genomes. The virus does all of its damage ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...the current Ebola crisis, Science and Science Translational Medicine have made their complete collection...

  13. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... strategy to enhance HDR repair using a single-stranded oligodeoxynucleotide HDR template covalently attached...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

  14. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...ample resources and a positive perspective on the transition to a post-academic career. This training was ... inadequate job of supporting science trainee transition into diverse, successful careers.  We’re not ...components of predoctoral and postdoctoral training. A grant from the National Institute of General Medical Sciences...

  15. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ... °C but preferentially nicks the non-target DNA strand. The lab then found gain-of-function mutations ...mutations that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature... of stable genomic integration and is easily transferred via conjugation.   Read the paper in Nature...

  16. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

  17. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental... but with the right incentives (for example, by grant agencies), we can shift toward a more sustainable...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

  18. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...connection that many people honor. Talking with strangers is usually challenging but does get easier with...you might spend a whole day having to talk with strangers. Be bold in asking all kinds of people to connect...to share their stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just...

  19. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ... LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic... a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in irradiated recipients...four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME Knocks out...

  20. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar....lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...
Showing: 521 - 540 of 809 results