...characterized by single, linear, negative-sense, single-stranded RNA genomes. The virus does all of its damage ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...the current Ebola crisis, Science and Science Translational Medicine have made their complete collection...

... strategy to enhance HDR repair using a single-stranded oligodeoxynucleotide HDR template covalently attached...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

...ample resources and a positive perspective on the transition to a post-academic career. This training was ... inadequate job of supporting science trainee transition into diverse, successful careers.  We’re not ...components of predoctoral and postdoctoral training. A grant from the National Institute of General Medical Sciences...

... °C but preferentially nicks the non-target DNA strand. The lab then found gain-of-function mutations ...mutations that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature... of stable genomic integration and is easily transferred via conjugation.   Read the paper in Nature...

...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental... but with the right incentives (for example, by grant agencies), we can shift toward a more sustainable...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

...connection that many people honor. Talking with strangers is usually challenging but does get easier with...you might spend a whole day having to talk with strangers. Be bold in asking all kinds of people to connect...to share their stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just...

... LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic... a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in irradiated recipients...four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME Knocks out...

...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar....lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...

...life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

...data, more discussion, and more functionality. Transparency, both in what works and in what doesn’t, and...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

...historians have the most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines...Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting...

...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...and gRNAs whose expression varies depending on transgene design and delivery. They generated hPSC lines...

... Today, that changes. Today, Addgene launches a brand new Europe office located in London and operated...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...

... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (...

...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...

...of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... Portugues, Ruben, et al. "Optogenetics in a transparent animal: circuit function in the larval zebrafish...

... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

...between my lab in Pennsylvania and a team in Paris, France. While efforts like these accelerate the progress...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide input...International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances permit...

...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...