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Showing: 521 - 540 of 803 results

  1. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...being plated on agar with ampicillin selection.  Notes and tips  We’ve found that our competent cells ...contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

  2. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ...

  5. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...combining fluorescent tags, epitope tags, protease sites, and selection markers. These are especially useful...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...gives a presentation on what they do and how it relates to other teams at the company. This provides new...old) employees with information on how Addgene operates as a team and shows how they fit into the system...

  9. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab repressed ctrA...that the levels of ctrA mRNA decreased after 20 minutes in xylose (which controls expression of dCas9)....

  11. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...

  12. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...

  14. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used...

  15. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor...

  16. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays...

  17. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make...

  18. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...

  19. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
Showing: 521 - 540 of 803 results