Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 867 results for: TOR

Showing: 521 - 540 of 867 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...unevenly, introducing distortions in your final images. It is possible to keep distortions to a minimum with...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to ...polymerizes, and the level of digestion. All of these factors in turn depend upon the specific gel recipe used... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength in the... forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires...that the protein could be taken directly from A. Victoria and expressed in any organism while still maintaining...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

  3. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...always get done, but you’ll have time to read a story to your baby or run that extra two miles which is...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...requiring either expressing it with additional cofactors or using significantly truncated forms that limit...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...much information as possible from depositing laboratories and associated publications, but our quality...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...user interface, so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for ...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS ...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...genetically encoded fluorescent probe. Several factors contribute to the popularity of GFP including (...organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter ..., proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains that...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein binds...Smaller genetically encoded probes are based on cofactor binding proteins as described above. The smallest...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record...means to manage their work, combining data, team, inventory and project management functionalities with experiment... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...equipment, Fluorescent emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...motifs within transmembrane proteins, such as receptors, or post-translational modifications on those ...) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells and...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note that when.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  11. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ... AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot ...AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional...Out All of Our Hot Plasmids Articles Browse the Repository  ...

  12. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

  13. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...

  14. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working with ...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844 . https... are even more exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of...breaks (Rees et al., 2018). For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window... guest blogger John Doench! John Doench is the Director of R&D in the Genetic Perturbation Platform at...

  16. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an Alzheimer..., the experimental group, with the new kinase inhibitor and a second group, the positive control group...protocol but does not receive either protein kinase inhibitor. Since the negative control group does not receive...If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better to treat...available materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit ...

  17. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...and viral vectors containing genetic elements from rabies plasmids to international requestors. We also...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other ...

  18. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one ... the scientists who deposited plasmids to the repository and for the scientific sharing community (check... in 2016 and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving...

  19. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...

  20. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...? 11:51 - 13:28 : How do you define what a good mentor is? 13:29 - 14:31 : What drives you to do science...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentorship... mentor...
Showing: 521 - 540 of 867 results