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We narrowed to 745 results for: TRI

Showing: 521 - 540 of 745 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...(deprotonated) forms of the chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

  7. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Abi run a Western blot and share tips for this tricky application.    You can check out all of our ...

  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

  10. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

  11. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

  12. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

  15. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...
Showing: 521 - 540 of 745 results