We narrowed to 746 results for: TRI

... that almost all off-target expression can be attributed to spontaneous recombination between homologous...the amount of leaky expression can largely be attributed to the level of spontaneous recombination between...

...This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene...

...(deprotonated) forms of the chromophore which contribute to the 2 peaks of wtGFP’s excitation spectrum...by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) the Youle Lab was able to ...

...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

... Abi run a Western blot and share tips for this tricky application.    You can check out all of our ...

...excited to share some good news with you: with contributions from depositors around the globe, we’re now ...

...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

...   “Unearthed Comics” by Sara Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman...

...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...

...Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. Agar art ...

...awesome new technology they’re utilizing, and the tricks they’ve used at their lab benches. It’s these exchanges...

...! AAV purification by iodixanol gradient ultracentrifugation This video goes over AAV vector purification...

...Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid...

...learned to prioritize projects, and how she sets metrics for success. The importance of thinking about the...