We narrowed to 754 results for: TRO

...system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external...spatial and temporal dynamics of apoptosis both in vitro and in living organisms to better study and understand...

...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...

...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

...sequence. Here at Addgene, we use NGS-based quality control to confirm the sequence of all the plasmids we ...ladder based on the expected band sizes. Always run control uncut DNA to ensure your enzymes are working. When...

...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

...already grown out of our workshops. If you're having trouble finding science communication opportunities in ...of its Leadership Team.  Nathan did his PhD in astronomy and works as a data scientist in the entertainment...

...Please keep in mind that this is just a brief introduction to the wide, wild, and fascinating field of ...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

... Image from Wen et al., 2017. Once you’ve introduced the reporter into the cells, you’re ready to ...sequence is also found in the reporter gene). A control RFP expression plasmid.  With this set up, all...

...Ginkgo Bioworks COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received...meant that we had to revamp some of our quality control processes. For the many other COVID-19 related ...

...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...

...presented Opto-CRAC which allows a researcher to control immune signaling and inflammatory responses remotely...deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics...

..., you’ll want the proper positive and negative controls for your PCR.  Find the details and learn more... this blog post for tips for analyzing and troubleshooting Sanger sequencing results. At Addgene, we now...

...improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without...domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu...

...employing a variety of cloning techniques and troubleshooting methodologies drew me to learn more about the...: Secondary Nanobody Toolbox Antibodies 101: Introduction to Antibodies Resources on Addgene.org  Browse...

...base pairing between these ~4 base overhangs isn’t strong enough to hold a plasmid together through transformation... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...

...minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology...assays for mutation screening Now that we have introduced how digital PCR works, let’s explain what kind...

...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...

...operon.   Tips for blue-white screening Use a good control: Transform the backbone plasmid without insert....reproduce to form colonies (used to complement auxotrophic cell lines), or genes which rescue a lethal phenotype...

...other than to sustain those techniques. We feel strongly that the scientific and the agricultural community...aesthetics with this technology and giving consumers control over their interaction with it, we hope to inspire...

...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...small insertions to allow in-frame deletions and introduce epitope tags and RNA transcription start sites...