We narrowed to 642 results for: VARS

1. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...impact your career, 2) given you tips for using various social media platforms, and 3) helped you fit social...

2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ... Various Addgenies...

3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...Nanobodies are a small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components...

4. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ... fragmented DNA released into the environment. Various factors promote natural transformation in different...

5. AAV Q&A with Tim Miles

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   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ...more information about PHP.eB brain labeling at various doses.  As always, you will need to discuss injection...

6. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...DNA can then be isolated and studied through a variety of downstream methods, such as qPCR or next-gen...

7. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...doctoral candidate in the Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources...

8. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ...will have three or more concurrent projects in varying stages, which can get messy if you’re not on top...

9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...your plasmid is often a numbers game. By using a variety of screening and selection techniques, you can ...

10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene...

11. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...displayed zero. Unlike Cas9, for which efficiency varies widely across guide RNAs, each guide tested with...

13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ... Cancer, Inflammation and Immunity, as well as various combinations thereof (so called “Immuno-Oncology...

14. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ... - SnapGene’s software seamlessly identifies a variety of common plasmid features such as antibiotic resistance...

15. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences in copy number...

16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...patient mutations using SDM can help determine if a variant is causal for a given disease. CRISPR has made ...

17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...limited packaging capacity. Still confused about the various tRNA expression strategies? No worries, I've summarized...

18. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

19. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to...

20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...the buck”. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...