...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

...are often used to study vertebrate embryology and cell development. One major resource for frog research...Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org...

...is immediately ready to transform into competent cells. The master mix of enzymes can be purchased from...mix for 1 hour, and then transform into competent cells. The success rate of this reaction is typically ...

...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology and scientific...

... mechanical method before your enzyme lysis. For cell cultures, enzyme lysis is typically sufficient.  ...proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, ...while semi-dry transfers require a semi-dry transfer cell. Dry/electroblot transfers only work in the proprietary...expensive Nitrocellulose or low-fluorescence PVDF membranes Increased fragility (nitrocellulose) or increased...When choosing your lysis buffer, consider the subcellular location of your protein. Proteins located in...Membranes The two membrane options are PVDF and nitrocellulose. PVDF is a sturdy membrane that can be stripped...membrane, it also has higher background noise than nitrocellulose membranes. Pro tip! You can visualize proteins...

...Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025...

...limit of detection for arg1 expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal...

...tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific...

...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

...anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools were pivotal in carrying out...

...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...

...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

...a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is ...

...experiments to test nickase preferences using HEK293 cells. They started by comparing D10A and H840A total ...

... expression after AAV injection, what region and cell class ended up expressing your virus? If you were...

... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...