We narrowed to 804 results for: cha
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TypeBlog PostPublishedJune 30, 2015, 2 p.m....cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...
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10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....people like a good story and science involves characters – in this case you – on a quest to ask and answer... Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m.... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) ...within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....general comments and adjust them if my opinion changed after this more detailed look. Encourage... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8 Chakiath CS, Esposito D (2007) Improved recombinational... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m.... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of...