We narrowed to 811 results for: cha
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TypeBlog PostPublishedOct. 18, 2017, 1 p.m.... a job and I stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or ...company. And make sure to subscribe to the Career Channel of the blog so you don’t miss the future posts ...
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Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m.... Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia,... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m.... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....localized, and how its localization and expression may change across various conditions. However, this system...Improved brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....people like a good story and science involves characters – in this case you – on a quest to ask and answer... Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m.... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8 Chakiath CS, Esposito D (2007) Improved recombinational... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations...