We narrowed to 763 results for: ire;

1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

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   June 30, 2020, 1:15 p.m.

   ...audience engaged. They first sent a pre-workshop questionnaire to the participants to learn about what they...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...

2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   Blog Post

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   June 23, 2022, 1:15 p.m.

   ...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

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   June 1, 2021, 1:15 p.m.

   ... research projects to a halt. None of us works directly with viruses or transmissible diseases, so we ...and availability; 3) Make the contact to start a direct collaboration between researcher and expert; 4)...

4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

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   Sept. 3, 2019, 1:15 p.m.

   ...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...such as transcription factors tethered to dCas9, require long time scales with constitutively expressed ...

5. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

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   June 4, 2019, 1:02 p.m.

   ...serotonin. Using a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput...anxiety, but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger...

6. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   Blog Post

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   March 30, 2023, 1:15 p.m.

   ... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

7. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

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   Sept. 19, 2017, 1:20 p.m.

   ...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

8. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

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   July 21, 2017, 12:48 p.m.

   ...tissue types! Although putting a gene of interest directly downstream of a tissue-specific promoter sounds...34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression systems...

9. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

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   March 17, 2020, 1:32 p.m.

   ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

10. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

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    Jan. 30, 2020, 2:15 p.m.

    ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

11. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

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    April 5, 2022, 1:20 p.m.

    ...to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

12. Antibodies 101: Isotypes

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    Blog Post

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    Oct. 19, 2021, 1:15 p.m.

    ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

13. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

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    Feb. 11, 2021, 2:15 p.m.

    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

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    June 6, 2019, 12:41 p.m.

    ...limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer. As...cell differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

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    May 3, 2018, 1:48 p.m.

    ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...

16. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post

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    Feb. 28, 2023, 2:15 p.m.

    ...clarified, especially with regard to licensing models. Direct sharing of work from universities and research ...Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying ...

17. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post

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    Jan. 21, 2025, 2:15 p.m.

    ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace... members thrived on independence, while others required more guidance and structure. I quickly learned...

18. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post

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    July 28, 2017, 1:30 p.m.

    ...same chromosome. Targeted Insertion allows for direct comparisons between lines expressing different ...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...

19. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

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    May 11, 2017, 2:30 p.m.

    ... contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of...Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer Program at the Broad Institute of...

20. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

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    July 28, 2015, 2:30 p.m.

    ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...