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We narrowed to 773 results for: ire;

Showing: 521 - 540 of 773 results

  1. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...optimized for your application. Depending on the tag, directly imaging or tracking your protein may also be possible...is not ideal with Myc, as low pH conditions are required during the purification, which may affect protein...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...large amounts of the target protein are typically required due to the relatively low efficiency of biotinylation... version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total amount...

  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...and control elements. Development of this tool required creating a database of common features, and devising...Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity when detecting a...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...: Do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...amplified (the ORF in this case), not the Tm of the entire primer. If you are amplifying from a plasmid or...

  7. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...ml This method is quick and simple and doesn’t require any special reagents. However, there’s limited ...absorbance-based methods, fluorescence-based methods require a standard curve, a set of samples with a known...

  9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...Berkeley’s Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct...need to get a handle on how cleavage sites are repaired.” Those insights will be critical for scientists...

  14. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... presence influences cell fates. The atlas of directed differentiation To understand how each human ...Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. 186 (1) 209-229. ...

  15. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...response, the variable region of an antibody is paired with any of the other isotype heavy chain constant...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

  20. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...new ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth...chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These...
Showing: 521 - 540 of 773 results