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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 855 results for: ple

Showing: 521 - 540 of 855 results

  1. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...cellular level. The reason for this is cost and complexity – anyone who works in the lab knows how expensive... engaging for students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in...educational activities using the BioBits platform, please get in touch with us! We’ll work with you to get...Addgene.org Browse our protocol videos to learn simple lab techniques Check out our molecular biology ...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...practical and hands-on. Students in these courses get plenty of theory in their lectures, but it can be difficult... format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease...modify BUILD YOUR PLASMID or generate games like it, please freely share your projects with us! This exchange...want to receive our modifiable powerpoint file, please feel free to contact us at: [email protected]...

  3. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...ul) samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP.... Likewise, a lot of scientists have requested samples of AAV for testing purposes. To address this, we... kit with all the serotypes, since these 20 ul samples are distributed individually. See our Serotype ...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...emotional time!” George Church tries to recruit people who are nice as top criterion and offers advice...productive in a handsoff environment. “Treating people fairly does not mean equally.” George Church was...comments that it is also surprising “ how easily people talk themselves out of great projects as ‘impossible...building up your lab and selectively picking the first couple of hires to build a strong, collaborative team....

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...for plasmid replication in mammalian cells, for example, and a toxin that kills bacteria may not have any... is possible that you may not want the plasmid depleted, but still want to use transient transfection ...HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes strand scissions 100-400 ug/mL...your cells divide. Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial...

  6. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...stable host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb...

  7. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...was not at all the case. The conference featured people who work in sociology, behavior, and artificial...versa. Even within the biological sciences, there’s plenty of interaction between neuroscience and other fields...microscopes, and dissection tools, but there were also people ready to discuss their VR helmets, gait tracking...field Not to toot our own horn too much, but many people who came to the Addgene booth (there were over ...

  8. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent protein...eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to bacterial membranes...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ...successfully launched, I would like to thank some of the people and organizations who helped us reach this milestone...donations. That seed money was used to build a completely self-supporting service so that Addgene is not..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

  11. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...primer known as a megaprimer. Piecing together multiple PCR products using sequence homology is also common... Cloning, and SLIC can all be used to assemble multiple fragments, but these methods can be expensive ...could make the E. coli do the work by using the principles of recombineering. In recombineering, bacteria...other methods, such “BAC shaving” would require multiple steps. One potential pitfall of REPLACR-mutagenesis...

  12. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then... factors can also influence NHEJ activity. For example, repressing RecA and overexpressing the NHEJ machinery...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...plasmids between scientists. The service would allow people to add a gene to a repository others could request... had 1,000 depositing scientists. Ensuring that people knew how to access Addgene, and that we were making...which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology ...reagents, and we realized we could use the same principles to provide recombinant antibodies, another plasmid-based...

  14. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to our Hot...aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows...

  15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...minutes of inactivating the CASANOVA complex, fluorescent CRISPR complexes were observed binding telomeric ...ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode ...2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012). The 2in1 Plasmid...

  16. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...accessible, anyone could present the material. For example, Addgene’s contributions by Tyler Ford included... and matched to serve a specific audience. For example, I joined this effort later in the year and in ...diverse and international team assembled the right people at the right time. Be inclusive and seek solutions...like to join or contribute to this collaboration, please check out the official website, follow the group...

  17. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...service, and more For the first time in the last couple of years, we don’t have to worry about having enough...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...unsolicited praise: “Addgene, for one, is a sterling example of collaboration and innovation between legal and...Our long reaching reputation helps us hire great people from farther away via connections and even via ...

  18. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...promoter region, which is predicted to be required to decouple gene expression and plasmid number. In a suite...fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before the...greatly impacting the level of gene expression. For example, the synthesis of deoxychromoviridans requires ...products is needed, such as within a multi-protein complex, stabilized promoters can eliminate variability...

  19. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... channels and pumps in developmental biology.  Examples of some ion-channel and ion-pump encoding genes...genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43...also modulating bioelectric states in vivo. For example, the Levin lab from Tufts University has used Arch...sensitive fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight ...

  20. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... when scientists talk about their precious RNA samples. RNA is inherently less stable than most macromolecules...RNA. To protect the RNA from degradation, several simple methods have been employed which modify the sugar...suggest coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications...RNA to control the entire editing system with a simple LED light. Lighting up your gRNA with fluorescent...
Showing: 521 - 540 of 855 results