We narrowed to 799 results for: por

...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101...

...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

...your data to a repository (Figshare is currently supported by ReFigure beta) you can include it in a ReFigure... ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering this...

...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust...

... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

...other areas of scientific research. We continue to support those involved in COVID-19 research, but our new...make science a better place. You will receive opportunities to collaborate with other scientists and to ...

...be shared with others. The Web Importer plug-in lets you directly import papers from web pages and academic...

...proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’...

... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...