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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 792 results for: por

Showing: 521 - 540 of 792 results

  2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  3. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

  7. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...assembly approaches, and opens the door for new opportunities in the field.  Gibson Assembly® is a registered...

  10. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

  14. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... combined with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target...

  15. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...the right candidate for the job. How can you incorporate the skills developed during graduate school into...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using... substrate is required for imaging, and it is important to verify that this substrate does not alter cellular...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...nuclease assays are completely “blind” to this very important difference (Figure 3). If you are interested...

  19. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  20. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...aging. For both a screen and a selection, it is important to clearly define what type of factor you are ...whole genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you ...
Showing: 521 - 540 of 792 results