We narrowed to 904 results for: CAN

... to our “real” work. However, a well-run meeting can have quite the opposite effect. A great meeting should... meeting. Follow the tips below to learn how you can run a top-notch meeting. Define the meeting Define...some other commitment. An announcement of purpose can help everyone to ‘land’ in the meeting and get focused... direction you’d like to pursue? Undefined scope can lead to long meetings spent on details that do not... sure to keep them manageable. At Addgene, teams can add as many points as they like to the written agenda...we like to send around a Google doc where people can make comments. At meetings, we just focus on the ...meeting (see “defining the scope” above) or if you can cut it down to thirty minutes. Keep it moving: ...

..., which can be difficult to predict. Slower Dephosphorylation Rates: FRET-based reporters can have slower...measuring kinase activity at the single-cell level can aid in drawing connections between signaling activity...spectral properties that limit which fluorophores can be multiplexed together. Challenging  to Design: ...cytoplasm and/or nucleus, while FRET-based reporters can be used to measure kinase activity in specific subcellular...and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work from the Covert...

... DNA strands - a double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases...identical to the original DNA sequence at the DSB, or it can introduce specific mutations into the damaged DNA...displaces one strand of the homologous DNA donor and can template repair off the other strand; this results... loop (D loop). The recombination intermediates can then resolve to complete the DNA repair process ...Homology directed repair pathways HDR mechanisms can be divided into two main camps: non-conservative ...presence of only one invading strand at a DSB that can be used for repair (a one-ended DSB). This single...HDR is a powerful site-specific knock-in tool that can be harnessed by researchers for many applications...

...your lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors ...cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein...syncytias that cause the cells to die. Cytotoxicity can also be cell type dependent: if a cell has few receptors...lentivirus (Crawford et al., 2020). That way, they can study the spike protein’s role in infecting cell ...methods.  In terms of preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists...cells and neurons. However, VSV-G’s broad tropism can be undesired for in vivo studies since there’s the...the potential for off-target infectivity as it can bind and transduce many cell types before reaching ...

...methods and machines one team can learn so linking together expert teams can push a scientific project to...too), we will often look for partnerships where we can leverage another organization’s expertise to our ...meeting people with different mindsets and approaches can truly broaden your mind. When I first began collaborating...Getting companies to come to these types of events can be very tough. First I needed to find the right contacts...guidance. The relationships you establish in this way can, of course, lead to jobs and even favors, but you...don’t forget about collaborations and projects you can do with others after work. Get sponsors for a training...accomplish with people at your organization. You can meet collaborators  for lunch, make a point of talking...

...control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR ...asymmetric core. Recombination between these sites can lead to excision, inversion, and translocation of...Continuous inversion of the DNA sequence of interest can lead to poor expression of genes within that sequence... for Cre and FLP function. Because recombination can only occur between target sites of the same sequence...recombinase - keeping Cre out until needed But what can be done when the recombination should only occur ... cortical neurons and their synapses.   How can you label a single neuron and its synapses in the...the living brain? Cells of a specific brain area can be transfected by in utero electroporation. To achieve...

...research as well. For example, CRISPRa can be used to find potential cancer drug targets by finding genes that...these can be difficult to construct and deliver when compared to gRNAs. Additionaly, cDNAs cannot be used... the potential to be used in therapeutics, as it can increase gene expression at certain loci to create...al., 2016). Additionally, CRISPR-based activation can be made inducible upon environmental triggers. This...This is done by creating a split dCas9 protein that can reassemble when exposed to specific UV wavelengths...under native conditions, the less activation you can achieve using a CRISPRa. Your gene of interest might... four guides directed towards that gene as there can be a large difference in guide potency. Thus, in ...

...that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they be...mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not significantly excite ...green and yellow fluorescent protein wavelengths, it can be imaged either with standard green fluorescent ...reduction in emission readout, respectively. mNeonGreen can be used as a fusion tag for a wide range of applications... other common fluorescent proteins and therefore can be used in immunoprecipitation experiments with other... mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or...

...2002) and many downstream complexes involved, it can be difficult for scientists to understand how GPCR...transducers (collectively known as the transducerome) that can be activated downstream of GPCRs that are not involved...by a GPCR on the cell’s surface into action that can result in the initiation of a vast number of signaling...downstream signaling pathways.  This dissociation can be measured using a technique known as Bioluminescence... a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio ...GFP2 emission to RLuc8 emission (see figure). What can TRUPATH be used for? The Roth lab used the TRUPATH...transducerome from different GPCRs. This collection can be used to study endogenous ligands, the pathways...

...against CRISPR positive plants, but screening these can be challenging and requires specialized equipment...mutant plants, but the seeds needed for experiments can only be collected from heterozygous individuals. Only..., T., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the mutations... (Top right, second panel) Transformed seedlings can be selected without antibiotics. (Top left) Mature...homozygous RUBY plant. (Bottom panels) RUBY expression can vary from different transformation events likely ...microscope. First, red CRISPR-positive transformants can be produced. In the following generation when CRISPR...to prevent further editing, then green offspring can be selected instead of red ones. Other innovative...