We narrowed to 614 results for: CaS;

...antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...pneumoniae, and P. aeruginosa) in addition to Vicrio casei, a bacteria isolated from cheese. Knockdown efficiency...promoters, selection markers, and different versions of dCas9. Following integration, Mobile-CRISPRi is stably...

...unusual blue-colored protein from the jellyfish Cassiopea xamachana (Blanquet and Phelan, 1987). Eight years.... Thus chromoproteins can be used as markers in cases where cost and resources are limited or for bioart... varied greatly between chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins...mesogleal protein from the mangrove jellyfish Cassiopea xamachana. Marine Biology 94:423–430 . https:/...

...systems, these systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there are no suitable...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...use the Cre/Lox system described earlier. In this case, you design your targeting vector such that a set...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

...measure how light travels through a sample — in this case, the absorbance of light at three key wavelengths...residual guanidine thiocyanate. Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and ...fluorescence based assay or running an agarose gel. In the case of plasmids, the use of RNase treatment to degrade...forgotten).    Improving read accuracy    In many cases, small amounts of contaminants in your sample will...

...tested four serotypes containing an expression cassette with an ubiquitous promoter to express GFP in ...in a group of young rats. We found that, in this case, AAV1 (23%) and AAV6 (<5%, data not shown) were not...Addgene has shown me this was far from an isolated case. Our scientific support team answers hundreds of...But, especially for AAV data, that may not be the case. We can all acknowledge that negative data is something...

... a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...miniCRISPR” version, are much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...

...for advice before running your analysis. In this case, I and other lab members had worked with lymphocytes... the positive one (Figure 8). Note that in many cases a stain will not result in clearly distinct positive...will show you a combination of two stains, in this case through conjugated antibodies targeting the cell...use multiple individual gates. Note that in this case, square gates were used.  Congratulations! ...

...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...eliminating two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once...

...Affordable, low-tech CRISPR/Cas9 experiments By Ashley Waldron CRISPR/Cas9 has revolutionized biotechnology...chromoprotein and gRNA plasmids. Nuclease-dead Cas9 (dCas9) protein is supplied separately. Results can ...

...Rosario F. Godino, Rocío López-Diego, & Zafira Castano Corsino. When someone says “internationalization...conceived and developed by its Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical...from the International Mentorship Program Zafira Castaño Corsino is a Junior Faculty at Harvard Medical ...and mentoring activities. Follow her on twitter @zcastan or on linkedln.       Rosario Fernandez-Godino ...