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We narrowed to 928 results for: Gene

Showing: 271 - 280 of 928 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral...disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to the presentation... Shao, L., & Wu, W. (2010). Gene-delivery systems for iPS cell generation. Expert opinion on biological...a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors... the transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences...demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming....pluripotency: history, mechanisms, and applications." Genes & Development 24, no. 20 (2010): 2239-263. PubMed...

  2. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

  3. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  4. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an ...3rd generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It...packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope...doxycycline to the grown media resulting in target gene knockdown. Note: this plasmid was called pLKO-Tet-On... 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...

  6. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday... to have many of our remote workers travel to Addgene's headquarters in Watertown, Massachusetts, for ...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...  Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some...including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable collection of memorabilia...our co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and...

  7. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...content collections is our Help Center, help.addgene.org, where you can easily search articles answering ...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...relevant in this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate...modifications.   Site-directed mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers...also generates 4 bp overhangs. You can then use the Klenow fragment of DNA polymerase I to generate blunt...Series Use CRISPR to Generate Specific Genomic Mutations Resources on Addgene.org Browse Our Molecular...Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used...analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution of key residues), and...

  9. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...work with fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. .... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... caters to optogenetics users and adjusts the lasers appropriately (tuned for optogenetics). These lasers...answer a research question. The flip side of the optogenetics coin is materials science-based. Light is delivered...optics is probably the least difficult portion of optogenetics (though it does require a significant amount...become go-to providers of materials needed for optogenetics. There are several important considerations ...with fabrication of the fiber optics needed for optogenetics. First, the length of the fiber optic for the...laser output), 5ms “on”, 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator,...manipulation of the laser power source itself. Pulse generation (TTL pulse) is a standard electronics protocol...
Showing: 271 - 280 of 928 results