We narrowed to 432 results for: sequence

...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

...CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just want...localization, binding to the appropriate promoter sequence and activation of gene expression. In addition...

...signal sequence and to the mitochondria via citrate synthase’s mitochondrial targeting sequence. Under...

...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...

...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...

...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

...[2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the...genes not in this collection, they may clone DNA sequences for creating riboprobes and dsRNAs into PR-T4P...

...fortunate to hand-deliver the model, the following sequence of events plays itself out: The researcher breaks...along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between ...

...by gel electrophoresis and/or next-generation sequencing more appropriate? If you already have an end...and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target DNA reporter system...