We narrowed to 841 results for: Abo;

...These days, it hardly seems like we finish writing about one dual CRISPR-transposon system before another...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...the same as not being a bad manager (see cartoon above – what type of bad manager have you had?). You can...Managing scientists I should add a few special words about managing scientists because, let’s face it, we are...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-Red: You can’t beet it!)   About the Author  Andrew Willoughby is a graduate student...

...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

...the ABRC are here to support you! From questions about growing conditions, to guiding students through ...local science festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone,...

...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...us with AAV testing and recently had this to say about the service: "I'm excited that Addgene is now...other expression data. If you have any questions about the Viral Service or any of the materials we distribute...

... using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology. “Virus...have a growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our ...

...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...getting together and chatting with another person about their career path is a great start to a mentoring...Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor: The...

...populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions...of days. Addgene: Can you tell us a little more about this new two-color tool and what it can enable? ...

... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...