...important immune cell type, in more complex and physiological microenvironments. These screens have primarily...molecular identifiers, which can improve the statistical power of in vivo screens. Figure 1: Using...Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in improving CD8+ T cell...

...alternatives for some of the harmful chemicals in your lab. These chemicals are not only better for the environment... resources about this, and even has a list of biological samples that are stored long term at -70 °C. ...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ... Feng Zhang’s lab recently developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter...will help researchers map transcription factor localization and binding via ChiP-seq. Learn more about...

...papers detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific...online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks like and... aren’t enough. Scientists are sharing what they call “raw science” – data sets, code, and, I think it...

...protein present on the virion surface – making it critical for viral attachment and fusion. Once inside a...glycoprotein’s role in infection also makes it a critical component of vaccines and the target of neutralizing...established Ebola Genome Portal at the University of California, Santa Cruz, offers sequence data on five strains...

...multiple timepoints. When the target of interest is critical to cell survival or proliferation, an additional...a flow cytometry experiment. But validation is critical to ensuring accurate interpretation of your results...to start with a screening procedure in easily genetically modified cell types (top). The specific approach..., A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody validation procedure enables quantification...

...few years ago, researchers introduced what’s been called a major breakthrough in cloning technology with...cassettes onto a single T-DNA in subsequent rounds, dramatically reducing the time required to generate multi-construct...multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save you half a...

...promoter. It can be used to drive Cre expression specifically in neurons. AAV Delivered Cre - A useful tool...Indeed, this AAV provides you with a means to specifically activate expression of any Cre-dependent gene...Additionally, you could use the retrograde form to specifically express Cre in neurons with axons that extend...

...sequence in live cells. This technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing...

...meet interesting people doing interesting things. I call this building relationships because networking has...or a particular position at their company, do NOT call this an Informational Interview. If you do this,... if it is not possible consider a phone or video call. If you do decide to use Skype, make sure to follow...

...Science and Technology Policy issued a memorandum calling on Federal agencies with over $100 million in annual...promote open science, ensure good stewardship of clinical trial data, serve society and patients, and respect...patents in a case against Myriad, an organization called Free the Data launched a public database to gather...

...used a GFP derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent... mammalian cells, and it may find wide use in biological research. Photoconvertible fluorescent proteins... new transgenic line could avoid problems in localization that have been encountered by some investigators...

...research, notably determining the subcellular localization and the number of disulfide bonds in your protein...protein expression in systems other than E. coli, specifically the bacterium Bacillus subtilis and the yeast...may improve expression of proteins that natively localize there. pCri is both versatile and easy to use,...

...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS accessible...expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...

...hour long interview. I anxiously prepared for the call. The length of this interview was intimidating to...team and shows how they fit into the system. Specifically as a co-op, it is encouraged to explore different... a 9-5 As it turned out, Addgene uses something called “core hours”. Core hours consist of the time between...

...Institute partners with companies to provide technological resources for those studying rare disease. This...will use fluorescent proteins to monitor the localization of these genes, CRISPR to disrupt their function... University of British Columbia Department of Urological Sciences. Dr. Nabavi studies peritoneal mesothelioma...

...parts. Their efforts yielded a protein, which they call SpyLigase (Spy comes from the “S” in Streptococcus...able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice and small, just 13...this tool? Howarth: Polyproteins are useful for mechanical study of proteins by atomic force microscopy ...

...the collection without getting overwhelmed by its scale: Look at the mEmerald variants first, followed by...fluorescently tagged versions of many proteins known to localize to specific cellular structures.The Davidson lab...you’re not sure where your protein of interest localizes in the cell, use these markers as your guide. ...

...CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer sequences...involve different proteins, but many of them are called Cas, short for “CRISPR-associated.” The most famous...integration into the array, these sequences are called protospacers. Repeats: Bacterial DNA that appears...