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Showing: 541 - 560 of 780 results

  1. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based...visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play...

  2. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken Graham, Ph.D....

  3. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...training from an organization such as COMPASS. Although COMPASS has a special interest in ocean science...

  4. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  5. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized...

  6. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve been very... in our building that someone from a different company had tested positive for COVID-19 (it turned out...

  7. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...

  8. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody  It can get even more complex if you’re looking for proteins that are interacting...

  12. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

  13. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...targets.  Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as...

  14. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...evolutionary perspective brought by studies of comparative anatomy showed me that some of the most fascinating...

  15. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...the Rare Genomics Institute sponsors a global competition for researchers working on rare diseases, offering...paralysis (hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on...

  16. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...
Showing: 541 - 560 of 780 results