We narrowed to 812 results for: DES

...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

...first NIH grant that Addgene has received. Addgene prides itself on being financially self-sustaining through..., Addgene’s interim Executive Director. “NABOR provides a new model for the creation and acquisition of...

...such as broccoli, cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known...kicked off with the release of 20 education kits designed for use in K-12 and college-level instruction....

...one list can ever encompass them all. We can’t designate non-academic jobs as “alternative” anymore. ...Develop expertise areas quickly Jack/Jane of all trades More sources of training and resources within...

...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

...references and web links from my first post and start designing your first fly experiment! Many thanks to our...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

...seeking “public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

...some point. Usually it comes up when a researcher decides on a new project and begins by trying to reproduce...they really want to do is get the paper off their desk and move on. Nevertheless, editors can and do play...

...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

...to your community. For those of you who have the desire and ability to, some communities are looking for... results. Also consider using this framework to design your future experiments.  Use Addgene’s AAV Data...

...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...