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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 623 results for: Dos;

Showing: 541 - 560 of 623 results

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... become valuable tools for your research! If they do (or if they don't!), please consider sharing your...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

  10. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... spin columns you need to complete your research. Do you know of other nucleic acid purification methods...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...was actually engineered to target the CNS. How well do these AAVs transduce the CNS and PNS? Both plasmids...from. Additionally, AAV.PHP.eB was used at a lower dose than it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral...blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail are injected, by chance lots...the two green neurons near the arrows. When lower doses of virus are used in order to decrease the number...AAV.PHP.eB capsid. Both viruses were IV injected at a dose of 1x1011 vg per mouse and the level of transduction...AAV.PHP.S capsid. Both viruses were IV injected at a dose of 1x1012 vg per mouse and the level of transduction...-off transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...
Showing: 541 - 560 of 623 results