We narrowed to 629 results for: Dos;

...more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...

... GetSet Need a set of overhangs, stat? GetSet can do that for you, complete with predicted fidelity. You...

... pooled libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled...

...high-quality data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

..., Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes cavitation....27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and...

...ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based titering assays, ...determination of the median tissue culture infective dose (TCID50) (Zen et al., 2004).  To choose the optimal...

...evaluate tens of thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting...

...in the biosynthetic pathway. Why plant scientists do transient leaf transformations The development of...

...Hoshino and Fujii, 2009). Other forms of enChIP, that do not use dCas9, require Zinc-finger or TAL proteins...

...Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant...

...single molecules - something researchers often want to do at neuronal synapses for instance. Also these tags...

...systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host...