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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 839 results for: IGH@;

Showing: 541 - 560 of 839 results

  1. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

  2. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

  4. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually... <- Can move between the two (helps to know the right people) -> How can you make a decision about ...interests and passions is another great way to shed light on what paths to pursue. Spend some time really ...

  5. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

  6. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...scientists, MTAs are important—they guarantee certain rights and protections for depositor and requestor alike... must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...Keima accumulates in autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

  8. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

  10. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

  11. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic...channels. These features enable the fibers to emit light for optogenetic purposes, measure temperature, study...construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the...

  14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...to activate neurons and neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these...updated and added new content to our eBook. Some highlights of the 2nd edition eBook include: new chapters...

  17. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...determine genome-wide translational frequency. Although highly useful, this technique requires extensive sample... nucleus to bind cognate untranslated mRNAs. The high specificity and resolution of this system are achieved...SunTag Catch up on Your GFP Background Choose the Brightest Fluorescent Protein Resources on Addgene.org ...

  19. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...viral samples. Read on to learn how aqueous samples might be affected by dry ice, and specifically how dry... removing the caps, incubating the tubes in an upright position for 30 seconds, and then recapping the...to prevent any pH change, as we still observed a slight change in pH (from 7.4 to 6.8). It’s possible that...

  20. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...as virus? “Like all things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and...lentivirus, we are providing lentiviral preps containing highly requested gRNAs including those targeting EGFP,...library has retained gRNA representation, produce high titer lentivirus from the library, ensure gRNA representation...
Showing: 541 - 560 of 839 results