We narrowed to 846 results for: IGH@;

... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

...communicated the science in my blog posts. This post highlights several of these free tools which will hopefully... selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search for images “Labeled...

...endophyte screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone... India Dr. Mrinalini Nair, Associate Professor (Right), focuses on molecular biotechnology for improving... Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors that detect...

... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

...unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration checklist...and more efficient. Our original policy was straightforward, but cumbersome: depositors had to email us...

...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives or even...to leave a little space for the unexpected — it might just be the key to unlocking the next frontier of...

...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

... host-pathogen interface, previously developed a high-throughput neutralization assay to study the link...Pfizer or Modern vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization... KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG...

...scientists, MTAs are important—they guarantee certain rights and protections for depositor and requestor alike... must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements...