We narrowed to 890 results for: IND;

...could be managing a team, planning meetings, and finding the best way to organize your time. At a conference...social media, you could be sharing your research or finding opportunities to bring science to the community...you love, you have to create opportunities. If you find yourself asking, "Why doesn't this product/organization...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight ...

...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...group to get real feedback Write whenever you can, find supportive editors so your first drafts are always...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the .... For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal ...

...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...two most popular plasmids of the past 10 years. Induced Pluripotent Stem (iPS) Cells - 2008 was marked ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...methyltetrahydrofolate, which are universally abundant. Second, induction of transcription occurs within minutes of light...

...mechanism. The Cas13 effector binds the guide RNA (shown as mature crRNA) and binds the RNA target before initiating...often recruits the main effector complex after binding to a target. Class 1 is the most abundant of the... functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment by Cascade... large-scale clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is...

...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more...proteins (proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to...

.... Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein resources...the practicalities and considerations you may not find in the materials and methods sections of optogenetics...Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nature neuroscience. ...

...determine if there have been any early successes, and to find out what delivery techniques researchers are using...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...programming noob (like myself) can understand. You’ll also find actionable advice on how to give a great science... (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order here...

...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which particular...Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled Libraries for Your Research...

...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...

...out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers...easy to miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela... look the same in every lab, but the principles behind this technique are beneficial for researchers at...   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...

... Below Bauer and Canver discuss the motivations behind this research.   Using CRISPR/Cas9 for targeted...deletion, the nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the...from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank you to our guest... mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian...

...about how I could fill this time. I was relieved to find out that the two hours were split up between several... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...at Addgene. This training prepared me for the independent work on the QC team. I was responsible for helping...Addgene that means that it is possible to work the remainder of the three hours (outside of the core hours)...

...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience.... During transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction... the AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV ...our AAV guide Learn more about our viral service Find retrograde AAVs at Addgene ...

...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems...annual NIH progress reports information on the Individual Development Plans (IDPs) for the graduate students...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

...the paper is published, while giving you peace of mind that your materials will only be made public when...will help us ensure that plasmids don’t get left behind in this status — a win-win situation for all. ...distribution instead of risking leaving them on hold indefinitely. Of course, the fastest way for us to release...out as planned, but you still hope somebody will find the plasmids useful, we’re happy to distribute unpublished...

...nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and an affiliated...was able to integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST...tailored treatments to specific mutations require individualized optimizations, safety testing, and approval...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared...