We narrowed to 772 results for: IRE;

1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying...editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...the research is ongoing, but all registries do require that research plans become public eventually). ...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

3. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...thus HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target...or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown. Multiplexing...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer. As...cell differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ... contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of...Jess S. Boehm. Jesse S. Boehm is the Associate Director of the Cancer Program at the Broad Institute of...

6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...same chromosome. Targeted Insertion allows for direct comparisons between lines expressing different ...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...

7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...antibodies to recognize and bind transcription factors directly, but that’s a slow-going process and it doesn’...transcription factors. The second vector is a homology directed repair donor plasmid with homology arms and a ...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...screens that can be done with the libraries will require access to next generation sequencing technology...due to the size of the backbone, transformations require electroporation. References & Further Reading...

9. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...available for a large number of targets, and can require specific conjugates. And, of course, once you have...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison...

10. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...and the lab, you may not be able to carry out the entire problem solving process on your own, but you can...skillset is a part of your toolkit is important. Paired with your other skills, both technical and transferable...

11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...BioRender.com. Image from Haoyi Qiu.   He Tian: Directed evolution of biosensors He Tian (goes by “Tian...developed a video-based high-throughput screen for directed evolution of biosensors based on their dynamic...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires no additional chemicals or modifications. This...optogenetic tools can be difficult because this requires detailed structural knowledge of the proteins....

13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...already run several reproducibility workshops as Director of Scientific Outreach at Code Ocean and through...

14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...peptide derived from cytochrome p450, CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

16. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...targeting efficiency of only 27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells...NHEJ rather than HDR. Figure 1: Homology-directed repair in targeted zygotes proceeds from the maternal...

17. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

18. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open...’ comments, and author responses can be shared directly alongside the preprint while the work is still...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...ability to turn on genes at different times. It requires more complex synthetic networks. Addgene: What... with the software to tell you which parts are required to get the function that you want. The last few...

20. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...