Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 795 results for: LAT;

Showing: 541 - 560 of 795 results

  1. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...several genes previously not associated with ricin regulation, including almost all genes involved in the production... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...increased and decreased p-values of known ricin regulation genes compared to results generated using non-targeting...

  2. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...unlikely candidate. It was discovered as an antigen upregulated on activated lymphocytes and is commonly used...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...adeno-associated virus-PHP. B capsid fails to upregulate transduction efficiency in the marmoset brain...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...which is the most commonly used bacteria in lab manipulations. Table 1 has a detailed list of the bacterial...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp....

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...single transcriptional activation domain - VP64.  Later generations of activators include the SunTag system...

  5. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...cells with VSV-G pseudotyped virus requires the upregulation of LDLR expression using other methods.  In ...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–6178 . https:/...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you... 37 °C.     The new pEcCas/pEcgRNA system We speculated that the gRNA on pCas that is specific to the... in the lack of recovered transformants. Our speculation motivated us to update the pCas/pTargetF system...

  7. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...publications. The policy was not an overnight revelation, rather it was the result of consultations between...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...interfaces where the viewer is more likely to want to manipulate how they view the information. We are used to...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...the mRNA — the bound miRISC can simply block translation machinery. The composition of the RISC varies...MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org...M. A. (2021). DICER: structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists... who will be listening.  Are they early, mid, or late career?  Will many non-scientists be present?  Always...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy...are known to express your target or have been manipulated to overexpress it. For negative controls, use... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...

  11. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...speaker Paula Stephan did a fantastic job of articulating many of these problems and some solutions. The...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

  12. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...

  13. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...the right decision. If I’d spent my senior year isolating pheromones from various C. elegans mutants by ...course, many types of teams. Some teams may be relatively permanent; some teams may only need to exist ...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...

  14. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...extract light from a single, thin plane within a relatively thicker three dimensional sample (see Figure ...technique should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...optical sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-...

  15. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...sequence came from. Entering this information will populate a list of possible Entrez Gene matches to your...easier for other scientists to find information related to your plasmid. It is optional to include the ...correct Entrez Gene does not appear in the auto-populated list, try entering the gene's Official Full Name...

  16. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

  17. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...package with Addgene mugs and some delicious hot chocolate for the lab. Here are some of our 2017 contest...Thank you to everyone who participated and congratulations to our 2017 #Deckthelab winners. We hope everyone...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...Mugford (@samtmugford) December 3, 2021   Congratulations to all our winners, and a huge thank you to...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the...Tip” filter on the blog to keep up to date on the latest episode.  Have some suggestions for future tips...

  20. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...repository what it is. So we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel...developed with their protein design approach. Congratulations to David Baker, Demis Hassabis, and John Jumper...
Showing: 541 - 560 of 795 results