...longer RNAs (such as mRNAs for gene expression). Examples of commonly used RNA pol II promoters for Cas9...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol...

...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96...

... NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your research...endogenous protein tagging as it is only 19 kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178...Another challenge to using FRET sensors comes when employing optogenetic regulators to initiate the event being...

...engineered ESCs), and chimeric adults mated to produce completely transgenic offspring. Although powerful, this... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...enrichment and selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating...

...with and be inspired by the mentor’s successful example. For evidence of this, you needn’t look any further...cancer. She has guided me in my next steps. For example, how to get a fellowship to travel abroad, how ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...

...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

... favorite gene-of-interest, all the way to the complex multi-TU constructs that compose genetic circuits... backgrounds, MoClo can be invaluable for more complex editing and screening experiments in organisms ... blog Plasmids 101: Golden Gate Cloning High Complexity Golden Gate Cloning with NEB’s DAD New MoClo ...

...Watson & Crick - Science History Rap Battle A great example of science story telling following the unfortunate...science educator looking for creative ways to implement the next generation science standards and communicate...

...off their decorating skills. To join the contest, simply embellish and adorn your lab space or your work...imagination run loose on the lab. Have fun! Here’s an example from last year’s contest:   🎶Walking in a Winter...

...that within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the ...an account on PubMed. Once you have an account, simply click on your account name in the top right corner...

..., in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment... cells, an important immune cell type, in more complex and physiological microenvironments. These screens...

...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...recommended uses and education content. This work exemplifies our long-standing commitment to be responsive...

...Plasmids" from these citing articles. What's this? Simply put, if the authors of a "Citing Article" have ...find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in the ...

...mck-PGC-1a1 mice found it wasn’t that they were simply immune to stress altogether. Their muscles showed...muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette van Praag of...

...planning and execution. I am seeing many similar examples of science trainees taking a proactive role in... Consulting. I know of many schools that have completely student/postdoc-driven biotechnology clubs such...

...advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase reporters...swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited spectral...

... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ...

...metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...

... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in...

... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: OpenWetWare ...