We narrowed to 629 results for: Ncl
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TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....these solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems...
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Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....example of an individual part, this category also includes promoters, UTRs, terminators, etc. Once selected... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....selected by the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions are available to simultaneously... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....three or four base-pair sequences, and exclude or include specific overhang sequences, allowing it to be ... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....al., 2016). DREADD ligands Several DREADDs, including hM3D(Gq), have been derived from muscarinic receptors... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....replicating and spreading in non-target cells, including yourself as the researcher. Figure 1:... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....creating plasmids encoding affinity reagents. These include conventional heavy and light chain recombinant ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....promoters across these three species, the Roska lab concluded that a promoter’s ability to target a cell class... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....Twitter list contains >300 Twitter literature bots, including a bot for biosensor papers, microbe articles, ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m.... Find plasmids for other developmental models including flies, nematodes, and zebrafish Find plasmids ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....defined manner. This allows scientists to draw conclusions due to gene expression in a specific brain area... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....Split-BioID’s key advantage over other PPI methods, including BioID, is its spatiotemporal controlled labeling... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....your lab can do to get into the robotics spirit include: Start using electronic multichannel and single... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....Addgene’s CRISPR-based Protein Tagging collection includes systems for tagging mammalian cell lines, Drosophila... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....unfortunate experience of witnessing…nothing. For some unclear reason, cells are not getting transduced. Here,... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases. Figure ... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....High throughput transcriptome sequencing methods including work done by the ENCODE project consortium did... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....further the understanding of epigenetics; techniques include chromatin immunoprecipitation, fluorescent in-situ... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m.... at the Rockefeller University. His interests include the role of learning and memory in opioid addiction...