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We narrowed to 847 results for: PLE

Showing: 541 - 560 of 847 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...signals are integral to many facets of biology. For example, many heart issues stem from the heart beating ...which are electrical charges propagated through multiple cells to do things like translate perceived signals...naturally occurring voltage indicator. To give an example, a genetically encoded phosphatase (voltage sensing...then localize and emit signal from. This specific example is attractive due to the large number of sheer ...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...where the PhD application cycle becomes much more complex. “How do I reach out to potential mentors?” “Where...of schools by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research...right fit. Reaching out to PIs also means doing plenty of mini-interviews; these professors will not only...campus even if you can’t visit. By talking with people at the institution, you can get a better sense ...

  3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...alternative PAM sequences are longer and often more complex than NGG, resulting in lower overall genome frequency...According to this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins... and CAA PAMs, they saw that xCas9 3.7 cleaves multiple PAMs at a higher efficiency than SpCas9: NGT (...modifications that can be made to Cas9. It is a pleasant surprise that selecting for one beneficial trait...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...site destination vectors for inserting a tag at multiple target sites. SapTrap vectors can be easily modified...Cas systems used for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids - Protein... assay additional protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based...PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...selecting plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative to amp...host/plasmid DNA during selection if it’s not completely bound up. While this likely occurs at a low rate...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...way to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection...was also independent of another TTAGGG binding complex, shelterin. Swapping the DNA binding domain of ...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...that are sure to be enabled by this toolkit. For examples of the toolkit in action, see Cermak T, et al....

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... and the complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding.... Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both to...broadly useful vectors, a process that isn’t as simple as it seems. Take, for instance, the GCaMP-encoding...

  8. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...We think it’s so important that we have written plenty of articles on building successful mentor relationships...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...your mentor know what yours is! For instance, some people like to ask questions throughout training, while...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...tool is in such a small package. But don’t let the simple presentation deceive you; pooled libraries can ...visualization) that confirm diversity of the library sample, and any other information or scripts provided ...suffice…and the answer is no. Due to the size and complexity of pooled libraries, NGS is the best way to validate... plasmids didn’t amplify after your screen is completed!  Producing Your Lentiviral Library Once you've...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...Amplified nucleotides are combined with Cas 13a in complex with crRNA targeting the sequence of interest and...sequences from a population (either enriching or depleting specific RNAs out of a pool of RNAs) or studying...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...camera or tracking software. Locomotion is a highly complex process on the level of individual neurons and ...motivational and other aspects may be involved. For example, an animal moving around to explore the environment...the elevated plus maze, the light-dark box is a simple device comprised of a brightly lit (risky) and ...behavioral neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...https://doi.org/10.1126/science.aaq0180  Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao..., Nureki O (2014) Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell 156:935–949...

  13. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...only occur through editing by a APOBEC-Cas9n-UGI complex. So how exactly does this work? Figure 1:...DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create...DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction of ... single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...discovery can be hard since it’s frequently why many people get into science in the first place. The feeling...but for many graduate students and postdocs, the people you see in your bay for 8-12 hours a day become...worked at the bench for over 10 years, but when people ask me about my job, I have no qualms about saying...course, but you’ll inevitably have downtime and plenty of it. That’s not the case at an office job. I’...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences...that did the same thing? Make an effort to meet people. Attend smaller talks within the bigger conference...professionals, with the added benefit that most people at the fair will probably be willing to accept ...country; sometimes several per month, giving you a plethora of opportunities to explore! Again, attending ...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...become resistant somehow overtime. One fantastic example of intrinsic resistance would be Mycoplasma species...Figure 1): Over-prescription of antibiotics (for example, giving antibiotics for an infection that isn’t...treatment regiment might wipe out bacteria that are completely susceptible to a drug while a full course of ...likely to develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...are essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and...proved challenging to study. Cells often express multiple integrins that bind the same ligand, making it...development. Integrin structure and conformational complexity There are 24 known mammalian integrins, all heterodimers...demonstrated that anti-integrin αVβ5.9 “essentially completely blocks all adhesion,” inhibiting the function...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... into one of the loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (...into an intracellular loop of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, ...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...to the range of concentrations expected in your samples. Higher affinity isn’t always better, especially...

  19. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...or entire organism. Because each cell can have multiple copies of the transgene that will recombine independently... all cell types can be labeled, and that while multiple copies of the transgene can integrate into cells...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not... neurons over millimeter-long distances across multiple fields of view, whereas previous Brainbow technologies...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...plasmids with the piggyBac™ transposase itself, please read on to learn more about this exciting technology...also enables the excision of the transposon in a completely seamless manner, leaving no sequences or mutations...challenges still remain, such as making gene edits completely seamless (i.e., so no other genetic alterations...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...
Showing: 541 - 560 of 847 results