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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 787 results for: POR

Showing: 541 - 560 of 787 results

  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...from the mol bio side you’re sure to have many opportunities to learn about and develop technologies that...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...

  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers for postdoc...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target sites.) How...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways...chance of causing a frameshift mutation. Of some importance, the deletion can be less heterogeneous when ...

  5. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere...nutrient, the host cells will die unless they have incorporated the plasmid carrying the required gene.  The...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...where particular variants or mutations have been reported in people, we can use this kit to knock in those...

  7. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  9. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...identify highly biotinylated background proteins reported by other research groups that performed BirA*-...

  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...biology known as bioelectricity is revealing the important role of bioelectrical signaling mediated by ion-channels...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...for IgG subtypes from different animals so it’s important to choose the reagent that works for your species...sure the staining you are seeing is real. It’s important to include a negative control using serum from...

  12. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...combinatorial libraries, and high specificity has been reported for a number of targets. Due to their size and...ain't broke, don't fix it!  That being said, it's important to know all your options when planning and running...

  15. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...be helpful having a coach that can offer extra support for issues of diversity in science.  A great person...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  17. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Perturbation Platform, to produce their shRNA library to support functional investigations of the mammalian genome...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

  18. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...of AAAS, WCSJ or ECSJ. These meeting are THE opportunity to grow your network. Get to know and get known...or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship, which places...

  19. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...
Showing: 541 - 560 of 787 results