We narrowed to 599 results for: REV;
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TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....new knockout animal models in species that were previously difficult to engineer, like rats. If you have...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....faring with users in a minute, but, to start, let's review why NgAgo is in the spotlight and take a moment... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....exclusively to in vitro cell culture. The user should review the current literature about their preferred model... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....special? A major advantage of CRISPR/Cas9 over previous genome editing techniques is its simplicity and... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....ITAMs ensures effective negative selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m.... my presentation had only become public in the previous two weeks, which demonstrated to the students ... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....within mixed communities and into species not previously cultured or isolated (Rubin et al., 2020). ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....across regions. Our investigation of model fit revealed some potential instabilities in the China data... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis is performed ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....to make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....restricted transgene expression in the CNS and PNS Previously to broadly introduce a transgene into a specific... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....advantage of being directional, meaning that (in the previous example) one can control whether the third module... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....generalized expected results for the three primers previously described. When using insert-specific primers... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....join together to feast and play lawn games. In previous years, this event was held at Executive Director... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... duty for researchers when considering the peer-review system of journals and grant applications, for ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....strategies such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....Holmes, Christina, and William L. Stanford. "Concise review: stem cell antigen‐1: expression, function, and... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....skills are the non-technical skills you pick up wherever you work, and you’ve almost certainly developed... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....circumvent sequencing and the need for a DSB in their previous reporter, the Harris Lab created a panel of eGFP...