We narrowed to 837 results for: RING
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TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935...
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Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent...lines but reach their limits when it comes to transferring DNA into primary cells or non-dividing cells...virus. Even though this system was efficient at transferring the gene of interest to the appropriate cells... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying capacity...comparative study of strains of Halobacterium halobium differing in pigmentation." Biochemical and biophysical ...Schnütgen, Frank, et al. "A directional strategy for monitoring Cre-mediated recombination at the cellular level... -
Introducing...Addgene Quick Tips!
TypeBlog PostPublishedSept. 19, 2024, 3 p.m....pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench... Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...you all so much for an amazing twenty years of sharing science! ... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m.... What a fun video of Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019...snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters and Santa hats. There was even... -
Introducing Addgene's Microbiology Pages
TypeBlog PostPublishedJune 13, 2016, 7:08 p.m....Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ... -
Addgene’s New Way to Help You Share Science
TypeBlog PostPublishedNov. 14, 2013, 4:10 p.m....plasmid-based technologies. Commentary about scientific sharing and the open-access movement. At Addgene, we believe...the conversation! Do you have a question about culturing iPS cells? Confused by which system to use for... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog PostPublishedDec. 8, 2015, 3:30 p.m....and to get some insight into her management and mentoring styles. If you'd like to learn more about Professor...defect in a single gene) 26:00-28:39 : Methods for Delivering Multiple Genes to Cells for Gene Therapy 28:40... -
AAV Packaged on Request is Here!
TypeBlog PostPublishedJan. 10, 2025, 1:01 p.m....packaged on request, you’ll see a banner below the ordering table. Click on it, select your desired serotype...find this service useful in your research. Happy vectoring! ... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....vectors repository since March. Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru...PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants... -
Visualizing Your Plasmid Request Data
TypeBlog PostPublishedApril 11, 2023, 4 p.m....standardized way. Since Addgene encourages the public sharing of aggregated data only, information on requesting...you find the dashboard useful when analyzing and sharing your own depositor metrics! About the Author Joachim... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists Click ...synthetic biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog PostPublishedNov. 8, 2016, 3:30 p.m....direct impact on human health and look forward to hearing from her in the future. Future awardees We will...to learn more about future awardees and their inspiring research. Additional Resources on the Addgene... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on new data from the Kim and...concrete context. I recommend this post to anyone wondering if and how they can apply FRET in their own research... -
The Addgene Antibody Data Hub
TypeBlog PostPublishedSept. 12, 2024, 1:15 p.m.... your research, you've probably found yourself staring at a browser full of tabs, each open to a different... a report with a DOI for citation. If you’re considering requesting an Addgene antibody, the Data Hub ... -
500,000 Plasmids Shipped and Counting!
TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m....extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on... on our mission – documenting your plasmids, storing them, providing quality control and customer support... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....infected in the United States while caring for a patient, despite wearing protective gear, and now comes news... -
What's New in CRISPR - May 2019
TypeBlog PostPublishedMay 28, 2019, 12:49 p.m..... coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9...genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....proteins and chemical contaminants that have been used during the purification procedure. Good indicators of ...guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure 2: Use cloning...