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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 809 results for: Ran;

Showing: 541 - 560 of 809 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

  2. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...The system consists of dCas9, the mammalian transcriptional repressor Mxi1, and an optogenetic tool that...CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells Lindy...and gRNAs whose expression varies depending on transgene design and delivery. They generated hPSC lines...

  3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...historians have the most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines...Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting...

  4. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...data, more discussion, and more functionality. Transparency, both in what works and in what doesn’t, and...Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza, E., Croteau, J., Thakur, N., Jonigk, D., Davis...00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R...

  5. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ... Today, that changes. Today, Addgene launches a brand new Europe office located in London and operated...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

  7. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...between my lab in Pennsylvania and a team in Paris, France. While efforts like these accelerate the progress...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide input...International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances permit...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...or A:T->G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...

  9. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...of neuroscience. The larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends... Portugues, Ruben, et al. "Optogenetics in a transparent animal: circuit function in the larval zebrafish...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...better. More transcription factor binding sites in a promoter lead to higher transcriptional activation....for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (...

  12. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... research project establishes your professional brand. It offers colleagues insight into your interests...understand where the field is today? Have you talked to veterans in the lab or the field? What are possible pitfalls...how can you mitigate them? If you were to write a grant for this project, what would be your critical experiments...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

  14. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...sequences required for viral replication and transcription as well as reporter genes. To learn how you ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  16. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...components. Without proper processing, these small DNA strands can form concatemers in later steps. This new NEB...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  18. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...particular study, which includes comparing the transduction patterns of different AAV serotypes. To help... Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules is integral ...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

  19. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...difference between airborne and aerosolised droplet transmission and how different fabrics and mask designs could...

  20. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... out and onto the screen and transform your work into a paper or a grant. Here’s a list of tools and resources...in red, words with a medium frequency of use in orange, and common words with high frequency of use in...
Showing: 541 - 560 of 809 results