Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 847 results for: STI;

Showing: 541 - 560 of 847 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...handy guide from OpenWetWare for a more extensive listing of common E. coli strains. Increase the volume ..., based on determining the cell density or by harvesting and miniprepping at various time points. Measuring...Bacterial Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich...

  2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...

  3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used ...will want to invest some time in optimizing and testing the efficiency of CRISPR delivery. There are a ... at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter System: Can evaluate...

  4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making ...efforts to develop formal guidelines to answer this question. Until then, using multiple confinement strategies...effort to implement (depending on the species in question), why not use them whenever applicable?Against...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular...vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons. Insertion...(Fig. 3), multiplexed insertions into multiple distinct targets could be achieved (Vo et al., 2020). VchINT... editing. Leveraging a unique marriage between distinct biological elements, CRISPR-transposons excel ...

  7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...ll prevent binding to any target protein that is still on the inside of the cell. However, assuming you...hidden epitopes. However, keep in mind that you will still contend with many of the same drawbacks to each ...early steps don’t even involve antibodies, they are still critical to the outcome of your IF experiment and...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...PEG) mediated transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. ... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam resistance genes, again with very ...PubMed Central: PMCID PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering...

  11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University of Tokyo...early research career. I've found that research institutes in different places have very different cultures...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...A. Victoria and expressed in any organism while still maintaining fluorescence. The protein structure,...cytometry that separates mixtures of cells into distinct populations based on fluorescent signal. Thus,...

  13. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...tactics to plan your work and make this happen. Be realistic and: Your co-workers will learn to work within...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...faster, and more efficient than previous methods. Still, generating reliable results from CRISPR edit data...comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE analysis of...contact us at [email protected] if you have any questions, and check out the Synthego blog for more great...

  15. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin... serves as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB...to apply this system as an orally administered diagnostic that can indicate the presence of liver metastasis...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are then cultured and FACS...cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently labeled piggyBac vectors...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...

  17. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...replication, the negative regulation system cannot distinguish between the different plasmids. The concentration... plasmids from the bacterial pathogens Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis...) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Letters in Applied...

  18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...available to researchers around the world. While we still offer many resources for producing vectors from ...vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023, we shared 93,491...difficult as it may be to find answers to such questions, Addgene is deeply interested in pursuing them...

  19. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...numerous fields including research and development, diagnostics, drug discovery, and food safety.  Part of the...antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that they do not ...is non-specific binding or false positives.  If testing a recombinant protein, especially one with tags...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...attention, though in reality much of our research still fails to reach our primary target audience – colleagues... disciplines and the years. Which leads to the question of how we can support researchers as they try ...much anyone applying the scientific method to a question. Videos from any discipline are welcome. Up to...
Showing: 541 - 560 of 847 results