We narrowed to 610 results for: STO;
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....encodes Cas9 and contains a site for inserting a custom gRNA. While this methodology has proven to be ... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m.....1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A, He C , Xu M, Zhou Z, Smock DC, Tang K, Owens... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m.... 2020. We fit our model for each region using stochastic gradient descent in Pytorch, which gave us the... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....complex biological reagents; many factors, like storage buffers and conditions (3,5,8), injection titers... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....becoming just the fifth repeat Nobel Prize winner in history. Interestingly, he also adds to the disproportionately... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....change your protein of interest. Fig. 2: Stop re-cutting with PAM and guide RNA disrupting mutations... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit.... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....you find yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....cultures of your positive clones. If you want to store your clones longer term, just streak the colonies... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....targeting a process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, Department of Microbiology... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....always get done, but you’ll have time to read a story to your baby or run that extra two miles which is... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I just had... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....post-injection. Livers from these mice were histologically normal and liver injury markers were not increased... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m.... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression...