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Showing: 541 - 560 of 797 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” or novel for their... understand the difference between the two, it’s time to check out our CRISPR library resources or our...

  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

  5. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

  6. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

  8. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...software tools for the right people at the right time. To explain a bit further, a software product manager...going to need to be a problem-solver. A lot of the time, the problem to solve is clear, but the solution...

  11. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...catalog are very well-characterized. During this time, we’ve also created a variety of educational resources...the foundations for many improvements to come. Ultimately, we would like our search engine to be so specialized...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest away the...-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works in your...

  16. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white...

  17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...

  18. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

  19. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...
Showing: 541 - 560 of 797 results