We narrowed to 807 results for: app
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TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....on OCRL1 already underway in his lab. The gene appears to play a role in many cell processes involving...In Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic...
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Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....would apply a percentage of returns to support student projects, but it quickly became apparent that raising... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing. Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....multidisciplinary, collaborative, and translational approaches for scientific tool and technique development...leadership, management, and communication. A great approach to fully understand and acquire competitive skill... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....years! This has helped us develop a fast, agile approach, which we’ve updated and refined as new technology...and our MTA YouTube video for more information. Happy transferring! Many thanks to our Tech Transfer... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m.... Another example of reagents that have broad applications but have proven useful to the study of SARS-...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....science. It's a series of case studies in what happens when scientists bring biases to research and don't...English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....exosomes, small membrane vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the... This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV antibodies... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....referred to objects that exist online, but this approach has been adapted for the identification of physical... Addgene plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....for additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be ...AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....assign each one using group members’ initials. If appropriate, email this around after the meeting. Accountability...Remember, many of the more in-depth discussions can happen “off-line” with just one or two relevant people... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence) to modify the desired genomic locus... more to be found in the full CRISPR 101 eBook. Happy Reading! ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your...culture to suppress bacterial growth, and is not appropriate for mammalian selection – do not confuse this... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....ll need a few controls specific to flow as an application. These controls will allow you to distinguish...isotype control. Pro tip! Many companies will have appropriate isotype controls linked on their commercial antibody... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m.... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m.... the appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....reactions by mixing this isolated plasmid DNA with appropriate sequencing primers, and hand deliver them to ...standards are met, we are taking a unique hybrid approach compared to our standard pipeline. For the first... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m.... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...faster deamination rate so that deamination can happen before Cas homologs let go. The Liu lab developed...