We narrowed to 665 results for: cell;

1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

2. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

   Type

   Blog Post

   Published

   June 23, 2020, 1:15 p.m.

   ...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

   Type

   Blog Post

   Published

   June 26, 2017, 1:31 p.m.

   ...tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific...

4. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

   Type

   Blog Post

   Published

   June 8, 2023, 1:15 p.m.

   ...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

5. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   Published

   June 10, 2014, 3:20 p.m.

   ...anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools were pivotal in carrying out...

6. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   Published

   April 15, 2014, 3:11 p.m.

   ...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

7. Tag Your Favorite Yeast Genes with Ease

   Type

   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...

8. Sensing Neuronal Dopamine

   Type

   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is ...

10. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ...experiments to test nickase preferences using HEK293 cells. They started by comparing D10A and H840A total ...

11. New Norepinephrine Indicators: nLightG and nLightR

    Type

    Blog Post

    Published

    Dec. 12, 2023, 2:15 p.m.

    ...al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before...

12. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    Published

    Sept. 12, 2023, 1:15 p.m.

    ... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...

13. Year of the Camelid: Antibody Style

    Type

    Blog Post

    Published

    May 9, 2024, 1:15 p.m.

    ...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size...

14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    Published

    March 5, 2020, 2:15 p.m.

    ... expression after AAV injection, what region and cell class ended up expressing your virus? If you were...

15. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ... results, but also confirm expression in target cells. While the various AAV serotypes can be used to ...

16. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...fellow at Vanderbilt University. She studies beta-cell function and failure in Type 2 Diabetes. Birmingham...BC (Canada). She works in a lab that uses yeast cells to study how DNA damage promotes cancer. She is ...

17. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ...recommend validating the serotype in your target cells before conducting the experiment. Likewise, a lot...

18. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...Associate at Harvard Medical School. He manages the Cell Biology Microscopy Facility, specializing in advanced...  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene...

19. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    Published

    April 23, 2020, 1:15 p.m.

    ... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ...

20. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321...