Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 811 results for: cha

Showing: 541 - 560 of 811 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is...

  3. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ...

  4. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...

  5. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative...

  6. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to...want to consider for future work placements. The chance to learn and collaborate all while experiencing...

  7. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (...

  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have...

  9. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA...extract DNA, sequence it, and analyze the data to characterize the microbiomes of the different samples. The...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

  12. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer...

  13. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...

  14. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically...gotten these services up and running. Both Alex Chavez and John Doench are members of the Addgene Advisory...

  15. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address...

  16. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...

  17. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...this into the agenda ensures that everyone gets a chance to talk and less vocal team members still have ...meeting a day in advance. This gives everyone a chance to review the agenda and add anything that is missing...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite...them in your experiments. Use the following flow chart to get you thinking about the types of experiments...

  19. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone...
Showing: 541 - 560 of 811 results