We narrowed to 710 results for: mak

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ... method overcomes many of these limitations and makes it easier for you to map protein-DNA interaction...

2. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   Published

   Feb. 1, 2018, 3:07 p.m.

   ...identify the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time...

3. Oh, The Places You Can Go: Careers in Science Communication - Product Development

   Type

   Blog Post

   Published

   Oct. 18, 2017, 1 p.m.

   ...like to work for a science education company. And make sure to subscribe to the Career Channel of the blog...

4. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   Published

   Sept. 14, 2017, 12:40 p.m.

   ...is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By mining the treasure trove...

5. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

   Type

   Blog Post

   Published

   Nov. 14, 2016, 3:30 p.m.

   ... tools with the scientific community worldwide, making it easier, cost effective and faster; this has ...

6. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   Published

   July 7, 2016, 2:30 p.m.

   ... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

7. Sensing Neuronal Dopamine

   Type

   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about...

8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...of course! You just need three plasmids to start making the AAVs you need for your experiment:  the packaging...

9. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...just Tris buffer, or some other variations. Does it make a difference? Short answer: yes. This last step ...

10. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    Published

    May 21, 2020, 1:15 p.m.

    ...labeling of a subset of cells with the same genetic makeup, overcomes all of these labor-intensive and invasive...

11. Hot Plasmids - October 2022

    Type

    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...photocurrents, and is more selective for K+ than HcKCR2, making it more suitable for in vivo optogenetic use.   ...

12. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    Published

    July 27, 2021, 1:15 p.m.

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type

    Blog Post

    Published

    May 2, 2023, 1 p.m.

    ...considerable distance away from a gene which can make identification of the gene itself difficult. The...

14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type

    Blog Post

    Published

    April 25, 2024, 1:15 p.m.

    ...Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned...

15. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...After joining a lab, the next important decision to make is “What project will I work on?” In the short term...

16. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    Published

    Sept. 18, 2018, 1:33 p.m.

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

17. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    Published

    Feb. 14, 2018, 2:36 p.m.

    ...template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this in the...

18. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...enhance protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because ...

19. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type

    Blog Post

    Published

    March 9, 2017, 3:40 p.m.

    ...helps to know the right people) -> How can you make a decision about something like this without sufficient...

20. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ... small nuclear DNAs from binding NgAgo, it might make it difficult to use NgAgo to disrupt multiple targets...